Journal: Neural Plasticity

Article Title: Transplantation of Neural Stem Cells Cotreated with Thyroid Hormone and GDNF Gene Induces Neuroprotection in Rats of Chronic Experimental Allergic Encephalomyelitis

doi: 10.1155/2016/3081939

Figure Lengend Snippet: Immunohistochemical double staining of BrdU, MAP2, GFAP, and GalC positive cells in the cerebral parenchyma of rats. (a–c) MAP2 − /BrdU + cells (solid arrow), MAP2 + /BrdU − cells (dotted arrow), and MAP2 + /BrdU + cells (arrowhead) in NSCs, T3/NSCs, and GDNF-T3/NSCs group, respectively. (d–f) GalC − /BrdU + positive cells (solid arrow), GalC + /BrdU − cells (dotted arrow), and GalC + /BrdU + cells (arrowhead) in NSCs, T3/NSCs, and GDNF-T3/NSCs group, respectively. (g–i) GFAP − /BrdU + cells (solid arrow), GFAP + /BrdU − cells (dotted arrow), and GFAP + /BrdU + cells (arrowhead) in NSCs, T3/NSCs, and GDNF-T3/NSCs group, respectively. (j) GFAP + cells (dotted arrow) in control group. (k) GFAP + cells (dotted arrow) in normal group. Bar (a–k) = 25 μ m.

Article Snippet: After a goat anti-mouse IgG secondary antibody (1 : 100; Wuhan Boster Biological Technology, China) was added for 30 minutes, alkaline phosphatase- (AP-) streptavidin (Boster) was incubated for 30 minutes, and 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium chloride (BCIP/NBT, Boster) was then used as a chromogen for 10 min. After PBS washes, the sections were reincubated with primary antibody for rabbit ant-MAP2, GFAP, or GalC, followed by incubation with goat anti-rabbit IgG (1 : 100; Boster) and horseradish peroxidase- (HRP-) streptavidin (Boster).

Techniques: Immunohistochemical staining, Double Staining

Journal: Neural Plasticity

Article Title: Transplantation of Neural Stem Cells Cotreated with Thyroid Hormone and GDNF Gene Induces Neuroprotection in Rats of Chronic Experimental Allergic Encephalomyelitis

doi: 10.1155/2016/3081939

Figure Lengend Snippet: Differentiation of NSCs, T3/NSCs, and GDNF-T3/NSCs in vivo (mean ± SD, n = 5).

Article Snippet: After a goat anti-mouse IgG secondary antibody (1 : 100; Wuhan Boster Biological Technology, China) was added for 30 minutes, alkaline phosphatase- (AP-) streptavidin (Boster) was incubated for 30 minutes, and 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium chloride (BCIP/NBT, Boster) was then used as a chromogen for 10 min. After PBS washes, the sections were reincubated with primary antibody for rabbit ant-MAP2, GFAP, or GalC, followed by incubation with goat anti-rabbit IgG (1 : 100; Boster) and horseradish peroxidase- (HRP-) streptavidin (Boster).

Techniques: In Vivo

Journal: Journal of Dental Sciences

Article Title: Overexpression of sprouty 1 protein in human oral squamous cell carcinogenesis

doi: 10.1016/j.jds.2020.07.013

Figure Lengend Snippet: Western blot analyses: phosphor-ERK and total-ERK in human oral cancer cell lines as compared with a primary culture of normal oral mucosa (HOK). Upregulation of phosphor-ERK (normalized to total-ERK) in OECM1 and Ca922 cell lines as compared with HOK, and a slightly decreased expression in SAS as compared with HOK. Results were quantified using densitometric analysis, normalized to the level of β-actin, and expressed as a fold change relative to the normal oral mucosa. Bars represent means ± standard deviation of the mean (∗ P < 0.05). A representative result of three independent experiments is shown.

Article Snippet: Further, samples were analyzed using 10% SDS-PAGE (Sigma-Aldrich) gels, and the proteins were transmitted onto a PVDF membrane (Sigma-Aldrich) using Bio-Rad's transblot with primary antibodies against phosphor-ERK (Boster Biological Technology, CA, USA; Cat. No. P00104; 1:1000) and total-ERK (Boster Biological Technology; Cat. No. P00104; 1:1000), with species specificity for human tissues and an observed molecular weight of 42–44 kDa; and β-actin (Sigma-Aldrich; 1:1000), followed by horseradish peroxidase (HRP)-conjugated secondary antibodies (Sigma-Aldrich; 1:5000).

Techniques: Western Blot, Expressing, Standard Deviation